Dados do Trabalho

Título

Effect of probiotic supplementation Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 on BDNF and intestinal parameters of sleep-deprived mice.

Introdução

Sleep deprivation can be a stress factor and leads to a decrease in BDNF (Brain-derived Neurotrophic Factor) levels, which has a neuronal protective effect in the brain, in the development of anxiety and memory loss. The perception of stress is linked to increased enteric inflammation, impacting the morphology of inflamed organs. The association of probiotics Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 has been studied as a possible therapeutic target through the microbiota-gut-brain axis, acting in an anxiolytic way and preventing hippocampal neurogenesis and changes in synaptic plasticity caused by stress.

Objetivo

To evaluate the supplementation of Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 on the effects of sleep deprivation on BDNF levels, number of enteric neurons and anxiety in mice.

Métodos

Male C57 mice (n=7-10/group) were used, divided into four groups: CTRL Group: No supplementation and no sleep deprivation (SD); CTRL-SD Group: Subjected to SD, without supplementation; Prob-CTRL Group: Subjected to probiotic supplementation, and without SD; Prob-SD Group: Subjected to probiotic supplementation and SD. Probiotic supplementation was started at 60 days of age, 5 days a week. After 6 weeks of supplementation, the Open Field (OF) test was performed to analyze anxiety levels, and then the REM sleep deprivation protocol began for 48 hours. OF was performed again after SD. At the end of the experiments, BDNF levels were analyzed in the hippocampus using the Indirect Elisa method. Jejunum samples were collected and subjected to the Indirect Immunofluorescence technique to highlight the population of total enteric neurons and the subpopulation of nitrergic neurons. All results were analyzed by one-way Anova (Post hoc tukey).

Resultados

The groups that received probiotics (PROB and PROB-PS) had an increase in BDNF levels compared to the control (PROB p=0.0005; PROB-PS p=0.0035). Furthermore, the PROB group demonstrated an increase in relation to CTRL-PS (p=0.0135). Regarding the number of total and nitrergic neurons in the jejunum, no statistical difference was found between the groups, as well as in the OF test (p>0.005).

Conclusões

Probiotic supplementation proved to be effective in increasing BDNF levels in the hippocampus of animals even in situations of sleep deprivation, demonstrating a neuroprotective effect. To date, no changes have been found in the population of enteric neurons in the jejunum.

Palavras -chave

Sleep; Microbiota; Hippocampus; Enteric Nervous System.